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    xmlns:ns5="http://nbrp.jp/ontology/"
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    xmlns:ns1="http://metadb.riken.jp/db/rikenbrc_mouse/"
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  <rdfs:Class rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC-AES1164">
    <ns6:brs_preservationStatus rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainStatus_4"/>
    <ns8:RO_0002162 rdf:resource="http://purl.obolibrary.org/obo/NCBITaxon_10090"/>
    <ns6:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_16"/>
    <ns5:organism rdf:parseType="Resource">
    </ns5:organism>
    <ns1:expectedDelivery xml:lang="en">C (3-6 months)</ns1:expectedDelivery>
    <ns6:brs_depositorOrganization xml:lang="ja">奈良先端科学技術大学院大学</ns6:brs_depositorOrganization>
    <ns4:application>Fluorescent Proteins/lacZ System</ns4:application>
    <dcterms:identifier>RBRC-AES1164</dcterms:identifier>
    <ns6:brs_depositor xml:lang="ja">石田　靖雅</ns6:brs_depositor>
    <ns1:ESCellLine>(C57BL/6J x 129/SvJae)F1 embryonic stem cell</ns1:ESCellLine>
    <ns5:organism rdf:parseType="Resource">
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    <rdfs:seeAlso rdf:resource="http://www2.brc.riken.jp/lab/animal/detail.php?brc_no=RBRC-AES1164"/>
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    <ns6:brs_depositedBy rdf:parseType="Resource">
    </ns6:brs_depositedBy>
    <ns4:termsAndConditions xml:lang="en">(1) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. When the RECIPIENT use the BIOLOGICAL RESOURCE in collaboration with a for-profit organization, it is recognized as a for-profit utilization and the RECIPIENT must reach agreement on terms and conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.&lt;br&gt;(2) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.&lt;br&gt;(3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR (Dr. Yasumasa Ishida ) is requested.&lt;br&gt;(4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of a paper (Nucleic Acids Res. 2005;33:e20) is requested.&lt;br&gt;(5) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.&lt;br&gt;(6) When the RECIPIENT established a mouse line by using of the BIOLOGICAL RESOURCE, the RECIPIENT must deposit the mouse line to the Experimental Animal Division of RIKEN BioResource Center.</ns4:termsAndConditions>
    <rdfs:label>24v-089</rdfs:label>
    <ns4:termsAndConditions xml:lang="ja">条件を付加する。&lt;br&gt;(1)　本件リソースの利用は学術研究機関の非営利目的の研究に限る。企業と共同研究を実施する場合は次項と同じ扱いとし、事前に寄託機関の承諾を得て、承諾書の写しを理研BRCに提出する。&lt;br&gt;(2) 本件リソースを非学術研究機関が利用することを希望する場合には、事前に寄託機関の承諾を得て、承諾書の写しを理研BRCに提出する。&lt;br&gt;(3) 利用者は、研究成果の公表にあたって、本件リソース開発者（石田靖雅博士）への謝辞を表明する。&lt;br&gt;(4) 利用者は、研究成果の公表にあたって、寄託機関の指定する次の文献（Nucleic Acids Res. 2005;33:e20）を引用する。&lt;br&gt;(5) 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請及び商業活動を行う場合には、寄託機関と別途協議を行う。&lt;br&gt;(6) 利用者は、本件リソースを用いてマウス個体を作出した場合には、当該マウスを理研BRCに寄託する。</ns4:termsAndConditions>
    <rdfs:label xml:lang="en">24v-089</rdfs:label>
    <ns4:addinfo>Necessary documents for ordering:&lt;ol&gt;&lt;li&gt;Order form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Category I MTA: MTA for distribution with RIKEN BRC (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Acceptance of responsibility for living modified organism (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;GFP Transfer License (&lt;A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude"&gt;English&lt;/A&gt;)&lt;br&gt;Please fill in the &lt;A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc"&gt;Schedule A&lt;/A&gt;, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read &lt;A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"&gt; Schedule B&lt;/A&gt;. &lt;/li&gt;&lt;/ol&gt;</ns4:addinfo>
    <ns4:addinfo>&lt;A HREF="https://getentry.ddbj.nig.ac.jp/search/get_entry?accnumber=CZ905121"&gt;DDBJ: CZ905121&lt;/A&gt;</ns4:addinfo>
    <ns6:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns6:brs_hasGenomicFeature>
    <ns6:brs_strainTypeLink rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainType_7"/>
    <ns4:resourceTypeLink rdf:resource="http://metadb.riken.jp/terms/xsearch#resource_type_Genetrap"/>
    <skos:altLabel xml:lang="en">Smarcc1 Gt/#24v-089</skos:altLabel>
    <ns1:expectedDelivery xml:lang="ja">C（3〜6か月）</ns1:expectedDelivery>
    <ns1:isRecombinant rdf:datatype="http://www.w3.org/2001/XMLSchema#boolean"
    >true</ns1:isRecombinant>
    <ns6:brs_strainTypeLink rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/straintype_13"/>
    <ns1:donorDNA>P1 Phage loxP, yeast FRT (flipase recombination target) sites, Jellyfish EGFP (green fluorescence protein) cDNA, mouse hypoxanthine-guanine phosphoribosyl transferase gene (hprt) genomic DNA, E. coli neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), LTR (long terminal repeat), SA (splice acceptor), CP (constitutive promoter)</ns1:donorDNA>
    <rdfs:seeAlso rdf:resource="http://www2.brc.riken.jp/lab/mouse_es/"/>
    <ns6:brs_depositedBy rdf:parseType="Resource">
    </ns6:brs_depositedBy>
    <ns1:strainDevelopment xml:lang="en">Developed by Dr. Yasumasa Ishida, Nara Institute of Science and Technology.  v6.4 ES cells derived from (129S4/SvJae x C57BL/6J)F1 were used.</ns1:strainDevelopment>
    <ns6:brs_hasAllele rdf:resource="http://metadb.riken.jp/db/mgi_rdf/MGI:4833356"/>
    <ns6:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns6:brs_hasGenomicFeature>
    <ns4:addinfo>Please contact "cellqa@brc.riken.jp" for distribution.</ns4:addinfo>
    <ns6:brs_depositor xml:lang="en">Yasumasa ISHIDA</ns6:brs_depositor>
    <rdfs:subClassOf rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/Strain"/>
    <ns6:brs_depositorOrganization xml:lang="en">Nara Inst. Sci. Tech.,Grad. Sch. Biol. Sci.</ns6:brs_depositorOrganization>
    <dc:description xml:lang="en">Gene trap ES cell lines using UPATrap method developed by Dr. Yasumasa Ishida, Nara Institute of Science and Technology.  UPATrap suppresses a nonsense-mediated mRNA decay of the selectable-marker mRNA and permits the trapping of transcriptionally silent genes without a bias in the vector-integration site.  These cells can be used in the in vitro differentiation study.  In addition, gene inactivated mouse line can be generated by using these cell clones.  Insertion of gene trap vector: pUPAT-3ic</dc:description>
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